Abstract:The analysis of genetic diversity among tomato cultivars was compared by simple sequence repeat （SSR） and morphological markers. Seven SSR primers were used to detect the genetic diversity among 11 tomato cultivars, and total 53 bands were obtained, the average bands per SSR primer pair being 6 with a range from 2 to 9. Mean genetic similarity coefficient among cultivars was 0.60, varying from 0.39 to 0.84. According to 11 phenotype values of morphological characters, the mean genetic similarity coefficient was 0.58, varying from 0.27 to 0.72. Cluster analysis by unweighted pair-group method with arithmetic mean （UPGMA） showed that both SSR and morphological markers could well distinguish the tomato cultivars by the colour of mesocarp and fruit size. The results of the evaluations of genetic diversity in tomato cultivars based on the two methods were similar.