Microdissection and Microcloning of Single Chromosome of Papaya

Abstract:

Single chromosome of papaya （Carica papaya L.） was isolated by glass needles, and the chromosomal DNA was amplified by LA-PCR （Linker Adapter PCR）. The PCR products were a smear ranged from 80 to 700 bp. Southern blot showed that the PCR products were homogeneous with the papaya genomic DNA, indicating that DNAs from the single chromosome had been successfully amplified. The PCR products were cloned into pGEM-T-Easy vector and about 1.18×10⁵ recombinant clones were obtained. The size of the inserted fragments of clones ranged from 100 to 400 bp.