Abstract:Twelve strains of endophytic diazotrophic bacteria were isolated from grain-straw-dual-purpose rice 201 popularized in Fujian Province. They were identified as： Enterobaeter cloacae, Enterococcus gallinarum, Bacillus purnilus, B. licheniformis, B. megaterium, B. sphaericus, B. cereus, Pirnelobacter, and Arthrobacter. Three strains with high acetylene reduced activity were selected to study the bacterial resistance to antibiotics, and results of Gram staining. The plasmids harboring the reporter gene lacZ fused to the promoters of nifH were transformed into Enterococcus gallinarum by triparental crossing. The inoculated rice roots were examined by β-galactosidase staining and observed under light and electron microscopes. The results indicated that the endophytic diazotrophic bacteria existed in epidermal cells, cortex cells, vascular tissue cells and intercellular spaces office roots.
