Abstract:The extraction of haemolytic toxin from Phaeocystis globosa was studied under laboratorial condition at various algal growth phases. Optimal conditions for breaking algal cell wall by ultrasonic was 600 W of power treated for 30 minutes at 4℃, the maximum volume of cultures being 3 000, 2 000 and 1 000 ml at logarithmic, stationary and senescent phases, respectively. High haemolytic activity was observed in stationary and senescent phases, but low or even nil in logarithmic phase. At least four haemolytic substances were found in this alga.