Abstract:Pollen of Torenia fournieri is two-celled and the division of generative cell occurs in the pollen tube. In vivo - in vitro technique was applied to get isolated sperm cells. After growing in vivo for 5 h, the pollinated style was cut and transferred into a medium containing 5% (w/v) sucrose, 0.01% (w/v) H3BO3, 0.01% (w/v) CaCl2, 0.01% (w/v) KH2PO4, at pH 5.0. Numerous pollen tubes emerged from the cut end of the style after culture for 2-3 h. When the pollen tubes were transferred to a broken solution containing 5％ mannito1, some pollen tubes broke and the tube contents including two sperm cells were released in the broken solution．The two sperm cells of Torenia fournieri are dimorphism：one is big and the other small．The bigger one (Svn) associates with vegetative nucleus and the small one (Sua) associates with the bigger one．Two sperm cells can be divided into two individual groups using a micromanipulator．These tw o dimorphic sperm cells could provide male gametophytic cells for further in vitro studies and various biotechnological experiments，such as preferential fertilization and gametic recognition in higher plant.