The Cloning of Replicase Gene of Banana Bunchy Top Virus and the Gene Expression

Abstract:

The DNA was isolated from banana grown in Guangzhou, which was infected with bunchy top virus. 1.1 kb DNA of replicase of banana bunchy top virus (BBTV) was obtained by PCR using this virus DNA astemplates for amplification. The derived DNA of the BBTV replicase was encoded by the C-Terminal of BBTV. It was homologous to 90% with the Australian BBTV. The reformed BBTV replicase was cloned to pBI 121 in the position between CaMV 35S promoter and NOS termination sequence, and a plant expressed carrier was established. Four transgenic platelets with BBTV replicase gene in T₀ generation were detected by PCR and Western blot analysis. The ability of these transgenic banana against bunchy top virus is being investigated.