Chromosome of Root Tip Cells in F_5 Plants Derived from Asymmetric Somatic Hybrids between Triticum aestivum and Agropyron elongatum

Abstract:

Asymmetric somatic hybrids having wheat appearance were obtained from protoplast fusion of Triticum aestivum L cv. Jinan 177 (as acceptor) and UV irradiated A gropyron elongatum (as donor) using PEG (polyethylene glycol) method. The karyotype analysis was made in root tip of F₅ of different lines (Ⅱ-2,8-1,Ⅱ-Ⅰ-8) from a somatic hybrid, and was compared with the parent wheat cultivar Jinan 177. The results indicated that the hybrids were basically stable in chromosome morphology and number. However, some differences in chromosome morphology were observed between the hybrids and the parent, as well as among the three hybrids, suggesting that small chromosome fragments from Agropyron elongatum were integrated into the chromosomes of the acceptor (wheat).