Abstract:The pollens of rice (Oryza sativa), maize (Zea mays) and Pennisetum alopecuroides were stored under low temperature (4℃) how humidity (RH 45%) , low temperature (4℃) high humidity (RH 90%), high temperature (35℃) how humidity (RH 45%) and high temperature (35℃) /high humidity (RH 90%) to determine the changes in proteinase activity, contents of proteins and free amino acids in the pollens. The results showed that the degradation of protein and the increase of the content of free amino acids were slow under low temperature and high humidity, but it was rapid under high temperature. Among the pollens of the three species, proteinase activity in rice pollen was high, and the degradation of protein contents and the increase of free amino acids were rapid. However, proteinase activity in pollen of Pennisetum alopecuroides was low, and the changes in protein content and free amino acids were slow. These changes in maize were intermediate between the above two species.