Abstract:Different volumes of Tris-HCl buffer (0.1 mol/L, pH 8.8) in combination with different contents of PVP40 were employed to study the embryo protein extracted from Litchi chinensis varieties "Huaizhi", "Guiwei" and "Heiye". It was shown that the volume of Tris-HC1 buffer being 5 times the fresh wieght of embryo and added with 15% PVP40 (g g-1 FW) was adapted to obtain a satisfactory result. Homogenate containing 15% PVP40 (g g-1 FW) could be used directly for determination of soluble protein content by Coomassie Brilliant Blue G-250 and 10% PVP40 (g g-1 FW) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Endosperm protein could be extracted with equal volume of Tris-HCl containing 2% PVP40. Precipitation of embryo and endosperm proteins with ethanol of 10 times the volume of protein extraction gave best SDS-PAGE pattern.
