Detection of Citrus Yellow Shoot Pathogen in Shatianyou Pommelo by Electron Microscopy and PCR Method
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    Abstract:

    Citrus yellow shoot disease (citrus huanglongbing) leaves of Citrus maxima cv. Shatianyou collected from pommelo orchard in Meizhou, Guangdong Province, showed typical symptom of leaf mottling. The pathogen which was observed under electron microscopy was similar in morphology and structure to bacteria-like organism of citrus huanglongbing disease. Polymerase chain reaction (PCR) analysis also proved that the DNA of the pathogen was greatly homologous with PCR product from that of citrus huanglongbing pathogen. The result exhibits that the citrus yellow shoot could infect pommelo, which seldom appeared in this fruit.

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梁炫强, 周桂元, 潘瑞炽.沙田柚黄龙病病原体的电镜观察与PCR检测[J].热带亚热带植物学报,2003,11(1):7~10

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