Induction of Solanum photeinocarpum Hairy Roots and Production of Secondary Metabolites

Abstract:

Genetic transformation of Solanum photeinocarpum by Agrohacterium rhizogenes R1000 and R1601． the effects of some factors on transformation efficiency and the production of secondary metabolites by hairy root culture were studied. Hairy roots were induced from leaf and stern explants with A．rhizogenes strain R1000 and R1601 ca. 7 days after inoculation Hairy root frequencies obtained from leaf and stem explants inoculated by R1000 harboring the agropine-type Ri plasmid pRiA₄b were 90.2％ and 50.0％ , respectively, while those by R1601 with the agropine-type Ri plasmid pRi1500 and pTVK291 were 92.1％ and 49.2％, respectively． Opine analysis indicated that T-DNA genes of A. rhizogenes strain R1000 and R1601 were expressed in S．photeinoccapum hairy roots. Hairy root-inducing frequency of leaf explants from S．photeinocarpum grown on MS medium supplemented with PP₃₃₃ was remarkably increased， and that of leaf explants inoculated by doubled diluting agrobacterium liquid on MS liquid medium was also increased． Hairy roots grew rapidly and the dry weight increased by 420 times after culture for 4 weeks， and the dry weight contents of total glycoalkloids and saponins were 3l and 107 times higher than those of the original ones，respectively．