Green globular bodies (GGB) first obtained from runner tips of Nephrolepis cordifolia Presl were cultured on MS medium supplemented with 4 mg L-1 BA and 0.1 mg L-1 IAA at 23 ℃ under continuous fluorescent light at 40 μmol m-2s-1 for 30 days. These GGB were then subcultured on the same medium every month. The differentiation and the growth of GGB subcultured on varies media or with different growth regulators, and various light quality and light intensity were examined and calculated after 70 days. The results showed that stimu1ating effects on the differentiation and growth were observed at irradiance of lO0 μmol m-2s-1．Red light was better for differentiation of GGB than blue or white light．In addition，MS medium supplemented with 1 mg L-1 6-BA and 200 mg L-1 NaH2PO4 was best for differentiation of GGB．