Abstract:Based on the 3'-end conserved regions among genomic RNA of tobamovirus, three primers P1, P2, P3 matching them were synthesized. ds-cDNA were synthesized to leading by these primers and cloned into the vector pBS. Fifteen positive recombinants were obtained after Hind Ⅲ + PstⅠ were digested. Among them, pBF3 was sequenced. It showed that the fragment of 2432 bp had two open reading frames (ORF). The first ORF encoded the frangipani mosaic virus movement protein. It ranged from nucleotide 806 to 1576. The deducing protein co nsisted of 256 amino acids．with a calculated MW of 28。5 kilo dalton．The second ORF encoded the coat protein which ranged from nuclco tide 1 635 to 2 158，and the deducing protein co nsisted of 1 74 amino acids，with a calculated MW of l9.4 kilo dalton． This frgment also included partial regions of 180 kDa protein and 3'-end non-coding regions．
