Abstract:A tissue culture system for obtaining high frequency shoot regeneration of Brassica campestris was studied. Shoots could be induced directly from cotyledons cultured on MS medium supplemented with 2.0 mg L-1 BA and 0.5 mg L-1 NAA, the shoot regeneration rate being 31.6%. Addition of 2.0 mg L-1 AgNO3 in the medium could greatly increase the frequency of shoot regeneration, and the shoot regeneration rate reached to 86.5%.