Abstract:Two Elephantopus species and one Pseudelephantopus species have been placed inone single genus Elephantopus L. sensu lato by some earlier systematists. The genomicprofile obtained by amplifying the DNA with different single primers were distinctive tothese three species and two genera. The Similarity Indexes indicated that Elephantopus mollisis more closely related to E. scaber than to Pseudelephantopus spicatus. The present studyindicated that the Elephantopus scaber L., and its closed species E. mollis as well as Pseudetephantopus spicatus were genetically to be distinguished． The estimates of genomic DNA fingerprints based on Similarity Index values ca lculated from amplified DNA band profiles show a dose association with known levds of morphological and histological， cytological relatedness．