PLANT REGENERATION IN FITRO FROM HYPOCOTYL EXPLANTS OF BRASSICA ALBOGLABRA

Abstract:

Plant regeneration from in vitro culture of hypocotyl in three cultivars of Brassica alboglabra was studied. The results showed that shoots could be induced directly from both ends of hypocotyl fragments with optimum auxins of 2.0 mg L^-1 BA for cultivar 'Early Flowering', 0.3 mg L^-1 NAA+ 2.0 mg L^-1 BA for' Middle Flowering' and 0.5 mg L^-1 NAA+2.0 mg L^-1 BA for' Late Flowering', the corresponding shoot-induction frequencies being 84.6%, 86.7% and 93.3%, respectively. The optimum concentration of sucrose for shoot-induction was 1%. Addition of 4.0 mg La AgNO₃ and 500 mg L^-1 MES in the medium could greatly increase the frequency of shoot regeneration. The plant let could well develope after rooting in half strength MS medium supplemented with 0.1 mg L^-1 NAA.No morphological differences were observed between in vitro plants and seedling plants.