Abstract:A simple method to create chromosome region specific library in plant materials,including microdissection, amplification, characterization and cloning, is described. A singleNOR region of chromosome I from Vicia faba was microdissected and directly amplified bySUP-PCR in a magnified system. The sizes of the PCR products ranged from 200 to 900bp. Southern hybridization with total genomic DNA demonstrated the reliability of themethod. Part of the products were cloned into plasmid PUC 18. The inserts ranged between0.25 and 0.9 kb.
