Abstract:Superoxide dismutase (SOD) extracted from mungbean (Phaseolus radiatus L.) seeds was entrapped with glycerin of various concentrations under various treatment conditions, then their stabilities were estimated. The results showed that the stability of SOD under hot stress, as well as, extreme acidic or alkaline stress was enhanced by glycerin entrapment. The concentration of glycerin was regarded as an essential factor for SOD stability, the optimum glycerin concentration was 12.5%～25%. It was demonstrated that 10℃, pH 6.8 and 1 000 r min-1 of mix round was the optimum condition to improve the SOD stability under hot stress, the half life of glycerin entrapment SOD in hot stress at 50 ℃ was 25.1 d, which was 5 times of the natural SOD not glycerin entrapment. Entrapment at pH 3.8 was more effective to improve the endurance of SOD in acidic stress，whereas, pH 8.9 was more resultful to enhance the stability in alkaline stress. Adding of Zn2+、Cu2+、Fe2+ was favor to enhance the stability of glycerin entrapment SOD under hot stress. Glycerin entrapment SOD also showed a rather strong tolerance in some additives of cosmetics. So glycerin entrapment would be a promising way to improve SOD stability in various stress conditions, which might enhance its various applications effectively in the near future.
