HISTOLOGICAL STUDIES OF PLANT REGENERATION MODES OF BRASSICA PARACHINENSIS BY AFFECTING AgNO3 AND ABA

Abstract:

Histological studies revealed that there were two different regeneration modes when cotyledon petioles of Brassica parachinensis as the explants were cultured in the medium with or without AgNO₃ and ABA. In mode Ⅰ,in medium with AgNO₃ and ABA,shoots regenerated directly from the cut end of cotyledon petioles and did not pass through callus stage. After 4 days of inoculation, the vascular parenchyma cells at the cut end of the explant initiated to active division and became meristem centre and then adventitious buds were regenerated. In mode Ⅱ,in medium without AgNO₃ and ABA, vascular parenchyma cells at the cut end initiated to division and gave rise to form root meristem or/and callus from which shoots and roots were subsequently regenerated. So the two modes of plant regeneration resulted in whether the medium contained AgNO₃ and ABA or not.