Abstract:There are growing evidences that the fertility of hybrid between indica and japonica varieties of rice (Oryza sativa L. )is controlled mainly by a wide compatibility gene (WCG), which is closely linked to the chromogen gene (C gene ). Screening new molecular markers nearby WCG and C gene by RAPD will be an effective approach to enrich the present molecular map. Some results obtained from RAPD analysis and bulked segregants of C gene for Near Isogenic Lines (NILs) of WCG are shown. By method of polymerase chain reaction (PCR), 22 polymorphic primers in 226 random primers are found for NILs of WCG, among which 20 polymorphic DNA fragments should be linked with WCG under theoretical calculation, and the expected minimum map distance is 0. 5 cent morgan. By using bulked segregants of C gene, 10 polymorphic DNA fragments in 200 random primers are found out.
