Abstract:Leaf-derived callus of Camellia sinensis Kuntze was obtained on MS medium supplemented with 6-BA (1 or 4mg·L-1 ) +IAA (2mg·L-1 ) +GA3 (3mg. L-1 ) +activated carbon (0. 2- 0. 3% ). On the same medium, embryogenesis was induced and finally formed plantlets. It was observed from the sections that the embryoids derived either from the cell or the cell mass on the surface of the callus, or from the cell or the cell mass in the deep inner pert of the callus. The developing order of embryoids was approximately the same as that of zygote embryos. They developed into plantletS via the stage of globular embryo, heart-shaped embryo, torpedo-shaped embryo and cotyledon embryo.Many abnormal embryoids appeared in the developing process.
