CLONING AND SEQUENCE ANALYSIS OF REPETITIVE DNA FRAGMENT FROM RICE ( ORYZA SATIVA L. ) A Proposed Role of Repeated DNA in Genome
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    Abstract:

    By application of appropriate vectors together with DNA renaturation kinetic method, repetitive DNA sequences of different Cot value were cloned from rice ( Oryza sativa L. ) genome. Two promoter selection plasmids, pKK175-6 and pKK232-8, were used as cloning vectors, which contained promoters Tet or Cat gene, respectively. The recombinant clones acquired resistance to Tc or Cm, and their resistant levels varied from 20μg/ml to 80μg/ml Tc or 30μg/ml to 260μg/ml Cm. The sequence analysis showed that there we...

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何木兰,唐东江,许莉萍,候永敏,张素敏,刘良式.水稻(Oryza sativa L.)重复DNA顺序的克隆及序列分析——重复顺序存在意义的探讨[J].热带亚热带植物学报,1993,1(2):62~72

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