Abstract:By application of appropriate vectors together with DNA renaturation kinetic method, repetitive DNA sequences of different Cot value were cloned from rice ( Oryza sativa L. ) genome. Two promoter selection plasmids, pKK175-6 and pKK232-8, were used as cloning vectors, which contained promoters Tet or Cat gene, respectively. The recombinant clones acquired resistance to Tc or Cm, and their resistant levels varied from 20μg/ml to 80μg/ml Tc or 30μg/ml to 260μg/ml Cm. The sequence analysis showed that there we...
