Abstract:An efficient method of callus induction and plant regeneration of Cuscuta chinensis was established. The seedlings germinated for 3~5 d and about 3~5 cm length were selected as explants, and then divided into three parts, upper, middle and lower. Callus were inducted from upper or middle parts of seedlings on a modified Murashige and Skoog (MMS) medium supplemented with 1 mg L-1 NAA and 1 mg L-1 BA. The calli have been subcultured on such medium for over a year. Shoot regeneration from callus was achieved on MMS medium containing 1 mg L-1 BA and could obtain 2.8 shoots per callus.