Pollen Morphology and Viability of Magnolia sieboldi

Abstract:

The collection, morphology characters and culture condition of pollens of Magnolia sieboldii were studied, and the effects of storage temperature and time on pollen viability were investigated. The pollen morphological characters and germinal aperture of Magnolia sieboldii under scanning electron microscope are similar to that of the other species in Magnoliaceae, but the size and exine sculpture are different. The pollen collected in the initial bloom stage has the highest germination rate at 25℃. Pollens cultured on medium with 10% sucrose and 0.1% boric acid for 14 hours had the highest germination rate. The 0.01%~0.05% CaCl2 had no significant effect on pollen germination, but 0.1% CaCl2 had negative effect. The viability of pollen of Magnolia sieboldii decreases gradually as increasing of preservation at different storage temperature, and the pollen viability are decreased in order of 25℃>4℃> -10℃. The pollen germination rate reduced from 87.9% to 48.8% when they were stored at -10℃ for 5 days and reduced to 0 for 10 days.

Key words:Magnolia sieboldii; Pollen; Pollen morphology; Viability; Storage