Abstract:cDNA of Jatropha curcas was amplified by PCR with degenerated primers, a new plasma membrane intrinsic protein gene (JcPIP) was cloned. Phylogenic analysis indicated that PIPs of Jatropha curcas was closer relationship to those in Ricinus communis, Vitis vinifera and Spinacia oleracea. The cRNA of JcPIP was hetero expression in xenopus oocytes as a water channel and increased swelling rate by 10 times. A 13 amino acid sequence peptide was selected from the hydropilic conserved peptides. Then a high titer polycolonal antibody was obtained by immunizing rabbits with the peptide. ELISA analysis showed the titer of rabbit serum against anti-peptide of JcPIP was up to 10-4. The location of JcPIP in Jatropha curcas was analysised with western-blot. JcPIP was major in 29 kDa band and located in all plant tissues. Treated with PEG-6000, the amount of JcPIP incresed, however, decreased under the conditon of rewater. It suggested that JcPIP related with drought tolerance of Jatropha curcas.
