Abstract:The volatile components extrected from leaves of Stevia rebaudianum Bertohi were studied using by Solid phase micro-extraction (SPME) and Simultaneous distillation extraction (SDE), and chemical constituents were analyzed by gas chromatograph/mass spectrometer (GC-MS). One hundred and one compounds were identified, in which there were sixty-seven compounds from SPME, eighty from SDE, among them forty-six from both SDE and SPME. The main compositions were trans-β-farnesene, β-pinene, caryophyllene oxide, spathulenol, nerolidol, which were much different from other Compositae plant.