Abstract:The effects of different freezing treatments on ultrastructure of plant cells during cryo-sectioning were compare. The results shown that the cell ultrastructure was well preserved with direct cryoembed method, while the endomembrane system in the cell was injured severely with liquid nitrogen cryopreservation. We proposed a method combining with cryoembed and modest thawing, by which not only could get integrated sections with the basic structure preserved, but also could avoid the abuse of using cyro-protected reagent. The procedure consisted of several steps as follows: fixing → refrigeration and embedding → modest thawing → rapid sectioning → section flatting → staining. The sections made by direct cryoembed with modest thawing could be stained and chemical determined in different ways. Besides, the characteristic of this procedure is simple and easy to be promoted.
