植物中的多酚物质对超氧物自由基的清除作用

THE SCAVENGING EFFECT OF PLANT POLYPHENOLICS ON SUPEROXIDE RADICALS

摘要:

芒果、番石榴、松、龙眼等叶片和绿茶中含有Ｏ２（超氧物自由基）的非酶促清除剂，仅０．５—１ｍｇ鲜重或０．２９ｍｇ茶叶就相当一个ＳＯＤ酶单位作用，热处理不能降低清除Ｏ２的能力．用显示酚类物质的喷洒剂（ＡｇＮＯ３－ＮＨ４ＯＨ）和显示ＳＯＤ同工酶带的ＮＢＴ法对电泳后的凝胶分别染色处理，对比显示结果，表明酚类物质与ＳＯＤ活性物质有相似的电泳行为，叶片中的酚类物质可能为非酶促清除Ｏ２的组份．人工合成的酚类化合物（对硝基酚、间苯二酚、愈创木酚）和从植物中分离的鞣酸等，在体外均能有效地清除Ｏ２．

Abstract:

The non-enzymic scavenger for superoxide radicals was found in leaves ofsome plants, such as mango (Mangifera indica),guava(Psidium guajava),pine(Pinusmassoniana) and green tea. The equivalent scavenging capacity for one unit of SOD activityin these plants was 0.5mg to 1.0mg of fresh weight or 0.29mg of dry tea. Heat treatment ofthe rude extract could not reduce the scavenging effect. Similar patterns onPAGE-electrophoretogram were identified either by normal NBT-SOD test or phenolicreagent(AgNO3-NH4OH), it indicated that phenolic compounds Aught be one of the maincomponents with non-enzymic scavenging effect on Moreover,some synthesizedphenolics (guaiacol,propyl gallate, p-nitrophenol)and plant digallic acid could asloscavenge the in vino.