乌龙岭’龙眼胚胎发育时期特异性蛋白质的变化

Changes in Specific Proteins in Embryos of Longan at Different Development Stages

摘要:

应用IEF-SDS-PAGE技术分析龙眼胚胎分化发育过程中蛋白质组分的变化。结果表明，在各发育阶段大多数蛋白质组分的电泳图谱基本一致，但也有变化。其中花后38 d存在TE₁(27.1kD、p，7.3)，TE₂(17.5kD、pI8.2)2个特异蛋白，45 d存在TE₃(11.4kD、pI7.6)，TE₄(13.2kD、pI9.9)2个特异蛋白，52d存在TE₅(22.6kD、pI7.2)，TE₆(18.6kD、pI8.3)，TE₇(23.5kD、pI3.6)3个特异蛋白。31 d胚胎电泳图谱中的蛋白质点数相对较多，表明此时蛋白质旺盛合成与积累，这与蛋白含量的变化基本一致。龙眼胚胎发育过程中特异蛋白的出现或消失．对胚胎的分化发育具有重要作用。

Abstract:

IEF-SDS-PAGE(isoelectric focusing electrophoresis-sodium dodecyl sulfate-polyacrylamigel electro-phoresis)technique was applied to comparing and analyzing the changes in protein component during embryo differentiation and development of longan (Dimocarpus longan Lout．CV．Wulongling)．The results showed that major components of proteins were generally similar at different development stages，but some changes also took place． The 7 new specific proteins were observed at different stages of the embryo development．They were TE₁(27.1 kD，pI 7.3)，TE₂(17.5 kD，pI 8.2)on 38 days after anthesis(DAA)；TE₃(11.4 kD，pI7.6)，TE₄(13.2 kD， pl 9.9)on 45 DAA；TE₅(22.6 kD，pI 7.2)，TE₆(18.6 kD，pI 8.3)，TE₇(23.5 kD，pI 3.6)on 52 DAA．These specific proteins showed good repeatability in the experiments． The number of protein points were comparatively higher on 31 DAA gel electrophoretogram． which indicated that rich synthesis and accumulation of proteins appeared at the stage of 31 DAA， and coincided with the results of the protein contents determined． The appearance or disappearance of the specific proteins might play an important role in longan’s embryo differentiation and development．