可乐茄叶肉原生质体培养再生植株

PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF SOLANUM QUITOENSE LAM.

摘要:

本文报道茄属果树可乐茄（ＳｏｌａｎｕｍｑｕｉｔｏｅｎｓｅＬａｍ．）叶肉原生质体的分离、培养及植株再生。幼嫩叶片原生质体经酶游离、纯化后，以１×１０４个／ｍｌ密度培养于稍加改良Ｋ８ｐ（附加２，４－Ｄ０．５ｍｇＬ（－１）、ＮＡＡ１．０ｍｇＬ（－１）和ＢＡ０．５ｍｇＬ（－１））的培养基中，三天后开始分裂，一周分裂３—４次。一个月形成小细胞团，植板率为０．１—０．２％，小细胞团转培养于ＭＳ＋２，４－Ｄ０．５ｍｇＬ（－１）上增殖后进行分化。原生质体来源愈伤组织在ＩＡＡ（０．１—１．０ｍｇＬ（－１））与ＢＡ或ＺＴ组合的培养基中能诱导器官发生，芽分化率最高可达４２．９％；但ＩＡＡ、ＢＡ、ＺＴ三者一起使用未见任何器官分化。小芽在ＭＳ＋ＩＡＡ０．２ｍｇＬ（－１）中生根成植株。可乐茄叶肉原生质体的植株再生，可应用于育种和茄属植物遗传工程研究。

Abstract:

A procedure for isolation,culture and plantlet regeneration from mesophyll protoplasts of Solanum quitoense Lain.was reported. Young leaf protoplasts from aseptic plants grown in controlled environment were plated at 1×104 protoplasts ml-1 on modified K8p medium with 2,4-D 0.5mg L-1+NAA 1mg L-1+BA 0.5mg L-1. Protoplast division was initiated after 3 days, and one week later the 3rd and 4th divisions were observed. After culture for four weeks the protoplast-derived calli grow to a size of 1─ mm in diameter, and the planting effeciency (PE) amounted to 0.1─0.2%. Protoplast-derived calli were transferred onto MS+2,4-D 0.5mg L-1 for proliferation. One month later the calli were transferred again onto MS+IAA+BA (or ZT). Subsequently, shoots occured at 42.9% effeciency on MS+IAA 1mg L-1 +ZT 5.0mg L-1. Shoots were rooted on MS +IAA 0.2mg L-1 successfully.