Abstract:In order to understand the function of anthocyanin from tropical orchid Dendrobium phalaenopsis, the anthocyanin of D. phalaenopsis ‘Sanya Sunny’ was extracted by ultrasonic-assisted method, the constituents of anthocyanin extract was analyzed by UPLC-QTOF-MS. The total antioxidant capacity (FRAP) and the scavenging capacity of diphenyl picroacyl (DPPH·) of the extract were determined, and ascorbic acid was used as a positive control to evaluate its antioxidant capacity. Rabbit skin irritation and eye irritation experiments were used to evaluate the irritation of extract. The results showed that a total of 15 anthocyanins were detected in the extract, such as aglycones include delphinium pigment, cyanidin, mallow pigment, petunia pigment and peony pigment. The extract has strong antioxidant activity in vitro. The scavenging ability of DPPH· and total antioxidant ability are both stronger than ascorbic acid at the same concentration. There were not irritation on rabbit eyes and skin treated with 0.25-1.0 g/L extract. Therefore, it was suggested that besides ornamental value, Dendrobium phalaenopsis ‘Sanya Sunny’ also had broad application space in medicine, food and cosmetics fields.