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曾丹琦1,2, 张明泽1,2, 何春梅1, 王浩斌1,2, 俞振明1, 司灿1,2, 赵聪慧1,2, 李冬妹3, 段俊1
1.中国科学院华南植物园, 广东省应用植物学重点实验室, 中国科学院华南农业植物分子分析与遗传改良重点实验室, 广州 510650;2.中国科学院大学, 北京 100049;3.顺德职业技术学院, 广东 佛山 528300
为了解铁皮石斛(Dendrobium officinale) WOX转录因子的功能,采用全基因组分析技术对铁皮石斛WOX家族成员进行鉴定,并进行生物信息学和表达模式分析。结果表明,铁皮石斛基因组中有9个WOX转录因子(DoWOX 1~DoWOX 9),大部分的DoWOXs含有2~3个外显子,启动子含有与激素诱导、逆境胁迫和生长发育有关的顺式作用元件。qPCR分析表明,DoWOX1DoWOX2DoWOX3DoWOX4DoWOX9在类原球茎中的表达量最高,DoWOX4在小花蕾中表达最高,随着花的发育,表达呈现下降趋势。此外,DoWOX3DoWOX7DoWOX9在合蕊柱上的表达量最高,而DoWOX4在唇瓣的表达量最高。因此,DoWOXs可能参与调控铁皮石斛的生长发育,且可能在维持类原球茎状态和花的发育中起重要作用。
关键词:  铁皮石斛  WOX转录因子  生物信息学  基因表达  qPCR
Identification and Analysis of WOX Transcription Factor in Dendrobium officinale
ZENG Dan-qi1,2, ZHANG Ming-ze1,2, HE Chun-mei1, WANG Hao-bin1,2, YU Zhen-ming1, SI Can1,2, ZHAO Cong-hui1,2, LI Dong-mei3, DUAN Jun1
1.Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Shunde Polytechnic, Foshan 528300, Guangdong, China
In order to understand the function of transcription factor WOX in Dendrobium officinale, the WOX family members in D. officinale were identified by whole genome analysis techniques, the bioinformatics of WOXs and expression patterns of WOXs were analyzed. The results showed that there were nine WOX transcription factors in D. officinale genome, named DoWOX 1 to DoWOX 9, respectively. The most of DoWOXs contained 2-3 exons, while DoWOX9 had only one exon. The promoters of DoWOXs contained cis-regulatory elements related to hormone induction, stress responsive, plant growth and development. The expression of DoWOX1, DoWOX2, DoWOX3, DoWOX4 and DoWOX9 were the highest at protocorm-like body (PLB) stage by qPCR, which of DoWOX4 was the highest at small flower bud stage, and then decreased. In addition, the expressions of DoWOX3, DoWOX7 and DoWOX9 were the highest in columns, and that of DoWOX4 was the highest in lips. Therefore, DoWOXs might be involved in regulating the growth and development of D. officinale, and play an important role in maintenance of PLBs state and flower development.
Key words:  Dendrobium officinale  WOX transcription factor  Bioinformatics analysis  Gene expression  qPCR

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