Abstract:The basic principle of chromosome sorting technology and the basic process of sample treatment were introduced. The chromosome suspension was prepared by synchronizing the apical meristem. And then the target chromosomes with high purity and high quantity were obtained by flow cytometry. The main applications of chromosome sorting technology in plants were reviewed, including the construction of physical maps, the development of DNA molecular markers, and genome sequence of the complex polyploid plant. With the continuous improvement and development of chromosome sorting technology, the development of probe markers for chromosome sorting and the optimization of chromosome DNA purification and amplification technology after sorted, it will provide more effective help for the genomics research of polyploid plants such as sugarcane.