19个枇杷杂交新品种(系)的SSR鉴定和指纹图谱构建

Identification and Fingerprint Construction of 19 New Hybrid Varieties (Lines) of Loquat by SSR

摘要:

为探究枇杷（Eriobotrya japonica）杂交品种真实性和DNA指纹图谱，对新育成的19个枇杷杂交品种（系）进行SSR标记鉴定分析。结果表明，从已发表的89对SSR引物中筛选出扩增条带清晰稳定的多态性引物19对，在24份枇杷材料中共扩增到83条带，每对引物平均扩增4.37条，PIC值为0.234~0.983，平均为0.764。经12对具有父本特征带多态性引物鉴定，19个杂交新品种（系）全部为真杂种，真杂种率为100%。UPGMA聚类分析表明，19个杂交新品种（系）的遗传相似系数为0.728~0.969，与杂交亲本‘新白2号’和‘贵妃’聚为同一个大类，并可细分为4个亚类，红肉与白肉的枇杷品种（系）间无明显划分。同时利用8对多态性SSR引物组合，构建了19个枇杷杂交新品种（系）的分子指纹图谱。这为枇杷品种鉴定、新品种权保护和杂交育种提供重要参考依据。

Abstract:

In order to understand the loquat (Eriobotrya japonica) hybrid authenticity and DNA fingerprint, 19 new loquat varieties or lines were analyzed by using SSR marker. Nineteen pairs SSR primers with clear and stable fragments were selected from eighty-nine previously published SSR. Totally, 83 amplified bands were detected from 24 materials of loquat, with an average of 4.37 for each primer pair. Polymorphism information content values ranged from 0.234 to 0.983, with an average of 0.764. The authenticity of all 19 new loquat varieties or lines were confirmed by 12 SSR markers with special bands of male parent, and the true hybrid rate was 100%. The genetic similarities among hybrids ranged from 0.728 to 0.969. The UPGMA dendrograms indicated that 19 hybrids could be get together into one big group (with the crossing parents ‘Xinbai No. 2’ and ‘Guifei’) and divided into 4 subgroups, and do not cluster by color of flesh. Furthermore, the DNA fingerprints for the 19 cultivars were established by 8 polymorphic SSR primers. So, these would provide references for cultivar identification, protection of new variety right and crossbreeding of loquat.