3种芽孢杆菌菌剂对望天树苗木生长和光合特性的影响

Effects of Three Bacillus Agents on Growth and Photosynthetic Characteristics of Parashorea chinensis Seedlings

摘要:

为加快望天树（Parashorea chinensis）幼苗的生长，采用随机区组试验设计，1.5 a生望天树实生苗分别接种胶质芽孢杆菌（Bacillus mucilaginosus）、侧孢芽孢杆菌（B.laterosporus）和巨大芽孢杆菌（B.megaterium）菌剂。结果表明，3种芽孢杆菌菌剂处理对苗木的生长、光合指标均有提高，其中苗高、地径增量分别较对照提高了30.2%~57.2%和5.3%~49.7%，根系活力提高了74.5%~227.4%，净光合速率提高了15.3%~227.6%。6.0×10⁹cfu/g的巨大芽孢杆菌处理的苗高、地径、生物量、根表面积、根平均直径、叶面积、叶绿素含量及净光合速率、实际光合效率、光化学淬灭系数均表现最好，与苗木质量指数和隶属函数法分析结果一致。因此，6.0×10⁹cfu/g巨大芽孢杆菌对望天树苗木生长发育的促进作用最大。

Abstract:

In order to accelerate the growth of Parashorea chinensis seedlings, the Bacillus agents and dosage were selected. The seedlings at 1.5-year old were treated with different concentration Bacillus agents, including B. mucilaginosus, B. laterosporus, and B. megaterium, respectively. The results showed that the growth and photosynthetic indexes of seedlings were improved by three Bacillus agents. Compared to CK (irrigated only with clean water), the height and diameter increment of P. chinensis seedlings increased by 30.2%-57.2% and 5.3%-49.7%, the root activity increased by 74.5%-227.4%, while the net photosynthetic rate increased by 15.3%-227.6%. The seedling height, ground diameter, biomass, root surface area, root average diameter, leaf area and chlorophyll content were the highest under the treatment of 6.0×10⁹cfu/g B. megaterium, as well as the net photosynthetic rate, ФPSⅡ and qP, which were consistent with the results of seedling quality index and membership function method. Therefore, Bacillus megaterium at 6.0×10⁹ cfu/g was the most beneficial microbial agent to improve the growth and development of P. chinensis.