重庆加工型辣椒种质资源抗疫病鉴定的分子标记筛选

Molecular Marker Screening of Processed Pepper (Capsicum annuum) Germplasm Resources against with Phytophthora capsici in Chongqing

摘要:

为筛选出适用于重庆加工型辣椒疫病抗性鉴定的分子标记，以63份重庆加工型辣椒种质资源为材料，研究了12个辣椒疫病抗性相关分子标记的筛选效率。结果表明，7个分子标记在种质间无差异条带，2个分子标记的扩增结果不稳定，只有ZL6203、ZL6726和E73等3个分子标记在种质间能扩增出差异条带。ZL6203筛选高抗、抗性和中抗材料的效率分别为87.50%、77.78%和63.64%；ZL6726筛选抗性和感病材料的效率分别为100.00%和66.67%；E73筛选抗性和高抗材料的效率分别为77.78%和62.50%。因此，同一辣椒抗疫病分子标记对不同抗性材料筛选效率存在较大差异，ZL6203、ZL6726和E73适用于重庆地区加工型辣椒抗疫病材料的鉴定。

Abstract:

To screen out molecular markers suitable for identification of Phytophthora capsici resistant pepper in Chongqing, the screening efficiency of 12 molecular makers for Phytophthora blight resistance was studied in 63 processed pepper (Capsicum annuum) germplasms in Chongqing. The results showed that there were no different amplification bands of 7 markers among the pepper germplasms, and the amplification results of 2 markers were unstable. Only 3 markers, such as ZL6203, ZL6726 and E73, could amplify different bands among pepper germplasms. For ZL6203, the efficiency of screening high resistant, resistant and medium resistant materials was 87.50%, 77.78% and 63.64%, respectively. For ZL6726, the efficiency of screening resistant and susceptible materials was 100.00% and 66.67%, respectively, and for E73, the efficiency of screening resistant and high resistant materials was 77.78% and 62.50%, respectively. Therefore, the screening efficiency of the same molecular marker for different resistance materials was quite different. ZL6203, ZL6726 and E73 markers were suitable for identification of Phytophthora blight resistant of processed pepper in Chongqing.