广东省猴耳环遗传多样性研究

Genetic Diversity of Archidendron clypearia in Guangdong Province

摘要:

为了解猴耳环（Archidendron clypearia）种质资源的遗传多样性，以广东省12个野生猴耳环群体的146份种质资源为材料，采用SSR分子标记技术对其遗传多样性和亲缘关系进行分析。结果表明，21对SSR引物共检测到249个等位基因，平均每对SSR引物检测的等位基因数（Na）为11.857，有效等位基因数（Ne）为3.500，期望杂合度（He）为0.718，多态信息含量（PIC）为0.676；12个群体中博罗群体的Shannon多样性指数（I=0.528）和有效等位基因数（Ne=0.716）均最大，是遗传多样性最丰富的群体；群体间的遗传分化系数为0.071，AMOVA分析表明，猴耳环的遗传变异主要在群体内（97%），群体内的遗传分化大于群体间。聚类分析表明，遗传系数在0.16时，可将12个群体分为6大类，与主坐标分析的结果大致相同。这为发掘、利用与保护猴耳环群体种质资源，开展猴耳环优良品种的遗传育种提供重要的理论依据。

Abstract:

In order to understand the genetic diversity of Archidendron clypearia germplasms resources, the genetic diversity and relatedness of 146 individuals from 12 wild populations in Guangdong Province were studied by using SSR molecular marker technology. The results showed that a total of 249 alleles were detected by 21 pairs of SSR primers with an average alleles (Na) of 11.857, the effective number of alleles (Ne) of 3.500, as well as expected heterozygosity (He) of 0.718, and polymorphism information content (PIC) of 0.676. The Shannon diversity index (I=0.528) and effective number of alleles (Ne=0.716) of Boluo population were the highest among 12 populations, which is the most genetically diverse population. The coefficient of genetic differentiation among populations was 0.071, and the genetic variation of A. clypearia was mainly within populations (97%), and the genetic differentiation within populations was greater than that among populations. Meanwhile, the cluster analysis showed that the 12 populations could be divided into 6 types according to genetic coefficient of 0.16, which was similar to that of principal coordinate analysis. So, these would provide an important theoretical basis for the exploitation, utilization, and protection of A. clypearia germplasm resources, and genetic breeding of fine variety.