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龙珠果茎段离体培养和组培苗耐盐性分析
刘艳艳1,2, 司灿2, 何春梅2, 俞振明2, 段俊2
1.广州花卉研究中心, 广州 510360;2.中国科学院华南植物园, 广东省应用植物学重点实验室, 中国科学院华南农业植物分子分析与遗传改良重点实验室, 广州 510650
摘要:  为建立龙珠果(Passiflora foetida)的快繁再生体系,以实生苗茎段为外植体,研究了植物生长调节剂对丛生芽诱导、壮苗生根的影响,同时对组培苗的耐盐性进行研究。结果表明,MS+6-BA 0.5 mg/L+NAA 0.05 mg/L培养基有利于诱导丛生芽并促进芽的生长;MS+6-BA 3.0 mg/L+NAA 0.3 mg/L培养基有利于诱导愈伤组织;1/2 MS+IBA 0.2 mg/L培养基适合小芽壮苗生根。组培苗移栽至泥炭土∶蛭石∶珍珠岩(2∶1∶1)的基质中,成活率可达92.6%,且植株生长良好。0~200 mmol/L NaCl处理的组培苗生长不受影响;超过200 mmol/L NaCl处理,植株出现矮化、叶片萎蔫、变黄等现象。随NaCl浓度升高,叶片的SOD活性逐渐升高,POD、CAT和APX活性则呈先升高后降低的趋势。这为龙珠果的种苗繁育、海滨生态修复提供了技术支持。
关键词:  龙珠果  茎段  丛生芽  愈伤组织  盐胁迫
DOI:10.11926/jtsb.4323
分类号:
基金项目:广东省林业科技创新项目(2015KJCX040)资助
Stem Culture in vitro of Passiflora foetida and Salt Tolerance of Seedlings
LIU Yan-yan1,2, SI Can2, HE Chun-mei2, YU Zhen-ming2, DUAN Jun2
1.Guangzhou Flowering Research Center, Guangzhou 510360, China;2.Guangzhou Provincial key laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
Abstract:  In order to establish a rapid regeneration system of Passiflora foetida, the effect of plant growth regulators on adventitious bud induction, rooting were studied by using stems as explants, as well as the salt tolerance of seedlings. The results showed that the induction and growth of adventitious buds were suitable on MS+6-BA 0.5 mg/L+NAA 0.05 mg/L medium; the callus induction was appropriate on MS+6-BA 3.0 mg/L+ NAA 0.3 mg/L medium; and the rooting of plantlets was available on 1/2MS+IBA 0.2 mg/L medium. The survival rate of plantlets could up to 92.6% after transplanted on matrix (peat:vermiculite:perlite=2:1:1). The growth of P. foetida seedlings was not affected under 0-200 mmol/L NaCl, while plants appeared dwarfing, leaf wilting, and yellow under more than 200 mmol/L NaCl stress. The SOD activity in leaves increased with increment of NaCl concentration, while POD, CAT and APX activities increased at first, and then decreased. These would provide technical support for the breeding of P. foetida and the restoration of seashore ecology.
Key words:  Passiflora foetida  Stem  Cluster shoot  Callus  Salt stress

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