青花菜BoSCL3基因的克隆和渍水胁迫下的表达特征分析

Cloning BoSCL3 Gene from Brassica olreacea var. italica and Expression Analysis under Waterlogging Stress

摘要:

为了解SCL3（scarecrow-like 3）基因的功能，从青花菜（Brassica olreacea var. italica）中克隆得到1个SCL3基因，命名为BoSCL3，其cDNA全长1 355 bp，编码446个氨基酸。BoSCL3分子量为49.96 kD，为疏水性蛋白，与油菜（B. napus）、芜菁（B. rapa）中SCL3蛋白的亲缘关系最近，同科植物的SCL3具有较高的同源性。荧光定量PCR分析结果表明，青花菜BoSCL3基因表达量随渍水胁迫时间延长先下降后上升，推测其可能参与渍水胁迫响应。这为探讨青花菜BoSCL3基因响应渍水胁迫的分子机制提供理论依据。

Abstract:

In order to understand the function of SCL3 (scarecrow-like 3) gene, a SCL3 gene was cloned from Brassica olreacea var. italic, named BoSCL3, which full length of cDNA sequence was 1355 bp and encoding 446 amino acids. The molecular weight of BoSCL3 was 49.96 KD, predicted to be hydrophobic protein. Phylogenetic analysis showed that the SCL3 in B. olreacea var. italica (BoSCL3) was closely related to those in B. rapa and B. napus, and SCL3 in the same family had high homology. The real-time fluorescence quantitative PCR analysis showed that the expression of BoSCL3 decreased at first and then increased with waterlogging stress time, indicating that this gene might be involved in response to waterlogging stress. Therefore, these would provide a theoretical basis for studying the molecular mechanism of BoSCL3 gene in response to waterlogging stress.