杭白菊花水提物的UHPLC-ESI-Orbitrap质谱联用分析

Chemical Constituents of Water Extract from Chrysanthemum morifolium Flowers by UHPLC-ESI-Orbitrap MS

关键词:  杭白菊  花  水提物  化学成分  液质联用分析

Key words:  Chrysanthemum morifolium  Flower  Water extract  Chemical constituent  LC-MS analysis

摘要:  为阐明杭白菊花水提物的药效物质基础，采用超高压液相-高分辨质谱联用技术对杭白菊（Chrysanthemum morifolium）花水提物的化学成分进行分析和鉴定。结果表明，从杭白菊花水提物中鉴定出376种化学成分，其中相对含量大于0.1%的成分有152种，相对含量较高的有绞股蓝皂苷XXVII、含羞草碱-O-β-葡萄糖苷、甲基-β-葡萄糖苷、d-1-O-甲基粘质肌醇、l（+）-甲基肌醇、过氧化苯甲酰、甲基-β-半乳糖苷、甲基-α-果糖苷、芒柄醇、松醇、（E）-2-己烯-α-阿拉伯糖-（1→2）-β-葡萄糖苷、米瑟毒苷等。杭白菊花水提物主要以黄酮类和生物碱类成分为主，分别有40和53种，相对含量分别为22.68%和26.57%，是杭白菊花发挥功效的主要物质基础。

Abstract:  In oreder to understand the effective substances of water extract from Chrysanthemum morifolium flowers, its chemical constituents were identified by high-pressure liquid chromatography-high resolution mass spectrometry. The results showed that 376 chemical components were identified from flower water extract, and the relative content of 152 components were more than 0.1%. The components with high relative content included gypenoside XXVII, mimosine-O-β-glucoside, methyl-β-glucoside, d-1-O-methyl mucoinositol, l(+)-bornesitol, lucidol, methyl-β-galactoside, methyl-α-fructoside, ononitol, pinitol, (E)-2-hexenyl-α-arabinosyl-(1→2)-β-glucoside, miserotoxin, and so on. Therefore, the maim constituents in flower water extract of C. morifolium were flavonoids and alkaloids, which had 40 and 53 kinds with relative content of 22.68% and 26.57%, respectively, indicating that the main material basis for the performance of C. morifolium flowers.