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三角梅cDOPA 5GT基因的克隆和光照对其表达的影响
刘姗, 孙蓉, 高静雷
攀枝花学院生物与化学工程学院, 干热河谷特色生物资源开发四川省高校重点实验室, 四川 攀枝花 617000
摘要:  为了解光照对三角梅(Bougainvillea glabra)葡糖基转移酶基因表达的影响,从其苞片中克隆了环多巴5-糖基转移酶基因(cDOPA 5GT)。结果表明,三角梅cDOPA 5GT基因的cDNA全长为1 446 bp,编码482个氨基酸,生物信息学分析表明cDOPA 5GT蛋白的等电点(PI)为5.77,具有糖基转移酶的特征基序,为酸性亲水跨膜蛋白,不含信号肽,二级结构以α-螺旋和无规则卷曲为主,氨基酸序列保守性较差。qRT-PCR分析表明,遮光处理cDOPA 5GT基因表达量和植株的甜菜红素含量均显著下降。这表明三角梅的色素合成受糖基转移酶基因调控,并与光照呈正相关关系。
关键词:  三角梅  葡糖基转移酶  基因表达  光照
DOI:10.11926/jtsb.4233
分类号:
基金项目:四川省国际科技合作项目(2019YFH0136)资助
Cloning of Cyclo-DOPA-5-glucosyltransferase Gene from Bougainvillea glabra and Effect of Illumination on Its Expression
LIU Shan, SUN Rong, GAO Jing-lei
Key Laboratory of Dry-hot Valley Characteristic Bio-Resources Development at University of Sichuan Province, Department of Biological and Chemical Engineering, Panzhihua University, Panzhihua 617000, Sichuan, China
Abstract:  In order to understand the effect of illumination on the expression of glucosyltransferase gene in Bougainvillea glabra, the cyclo-DOPA-5-glucosyltransferase gene (cDOPA 5GT) was cloned from bracts. The results showed that the full length cDNA of cDOPA 5GT was 1 446 bp, encoding 482 amino acids. The bioin-formatics analysis showed that isoelectric point of cDOPA 5GT was 5.77, showing an acidic and hydrophilic transmembrane protein without signal peptide. The cDOPA 5GT protein had characteristic motifs of glucosyl-transferase, and the secondary structure was dominant of α-helices and random coils, and the amino acid sequence was less conserved. The results of qRT-PCR and spectrophotometry showed that the expression of cDOPA 5GT gene and betacyanin accumulation in plants decreased significantly under shading by qRT-PCR and spectro-photometry. Therefore, it was suggested that the synthesis of betacyanin was regulated by glucosyltransferase gene, and positively correlated with light time.
Key words:  Bougainvillea glabra  Cyclo-DOPA-5-glucosyltransferase  Gene expression  Illumination

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