印度梨形孢对铁皮石斛种子萌发和原球茎生长的影响

Effects of Piriformospora indica on Seed Germination and Protocorm Growth of Dendrobium officinale

关键词:  铁皮石斛  印度梨形孢  种子  萌发  原球茎  生长

Key words:  Dendrobium officinale  Piriformospora indica  Seed  Germination  Protocorm  Growth

摘要:  为探究印度梨形孢（Piriformospora indica）对铁皮石斛（Dendrobium officinale）种子萌发和原球茎生长的影响，在铁皮石斛种子离体培养和原球茎生长阶段分别接种印度梨形孢，对其形态发育特征和生理特性进行研究。结果表明，接种印度梨形孢的铁皮石斛种子的起始萌发时间提前，接种印度梨形孢的铁皮石斛原球茎的株高、鲜质量、干质量、叶片数、叶长、叶宽、根数、根长等都高于对照。在接种60 d的种子萌发率为68.8%，高于对照（28.6%）；在接种80 d的铁皮石斛原球茎的叶绿素a、叶绿素b、总叶绿素含量显著高于对照，叶绿素a/b小于对照，净光合速率、气孔导度、蒸腾速率均高于对照，药用多糖含量显著高于对照。因此，接种印度梨形孢能促进铁皮石斛种子萌发和原球茎的生长，提高铁皮石斛的品质和产量。

Abstract:  In order to explore the effects of Piriformospora indica on seed germination and protocorm growth of Dendrobium officinale, the morphological and physiological characteristics were studied inoculated by P. indica on seeds and protocorm. The results showed that the initial germination time of D. officinale seeds inoculated with P. indica was earlier than the control, and the height, fresh weight, dry weight, number of leaves, leaf length, leaf width, root number and root length of protocorm of D. officinale inoculated with P. indica were higher than the control. The seed germination rate was 68.8% after 60 days of inoculation, which was higher than the control (28.6%). The chlorophyll a content, chlorophyll b content and total chlorophyll content in the protocorm of D. officinale inoculated for 80 days were significantly higher than the control, chlorophyll a/b was lower than the control, the net photosynthesis rate, stomatal conductance and transpirationrate were higher than the control, and the content of medicinal polysaccharide was significantly higher than the control. Therefore, the seed germination and protocorm growth of D. officinale were promoted by inoculation, and the quality and yield were improved.