安祖花胚性愈伤组织诱导及植株再生研究

Studies on Embryogenic Callus Induction and Plant Regeneration of Anthurium andreanum

摘要:

为构建安祖花（Anthurium andreanum）胚性愈伤组织再生体系，以3个盆栽品种幼嫩叶片和叶柄为外植体，分析了基本培养基、植物生长调节剂组合和培养条件等因素的影响。结果表明，安祖花胚性愈伤组织诱导的最佳培养基为改良MS₃+1.5 mg L^-1 2，4-D+0.5 mg L^-1 KT+4%蔗糖+2%葡萄糖+0.25% Phytagel，且胚性愈伤组织诱导能力差异显著，表现为‘粉冠军’ > ‘罗宾奴’ > ‘冠军’和叶片 > 叶柄，其中‘粉冠军’叶片的胚性愈伤组织诱导率可达57.9%。胚状体分化的最佳培养基为1/2改良MS_a+2%蔗糖+0.25% Phytagel，其中‘粉冠军’叶片诱导的胚状体分化率可达31.6%，且在光、暗下分化率的差异不显著。分化苗移栽后的成活率可达100%。

Abstract:

In order to construct regeneration system from embryogenic callus of Anthurium andreanum, the tender leaves and petioles of 3 cultivars were used as explants, the effects of medium, plant growth regulator combination and illumination condition on embryogenic callus induction and plant regeneration were studied. The results showed that the optimum medium for embryogenic callus induction was modified MS₃+1.5 mg L^-1 2,4-D+0.5 mg L^-1 KT+4% sucrose+2% glucose+0.25% Phytagel. The embryogenic callus induction rate had obvious difference among cultivars and explants, showing in the order of ‘Pink Champion’ > ‘Robino’ > ‘Champion’, and leaves > petioles. The embryogenic callus induction rate of ‘Pink Champion’ leaves could reach up to 57.9%. The optimum medium for embryoid differentiation was 1/2 modified MS_a+2% sucrose+0.25% Phytagel. The embryoid differentiation rate derived from leaves of ‘Pink Champion’ could reach up to 31.6%, and had not significant differences cultured under light and dark. The survival could reach up to 100% after transplanted plantlets.