Zn、B配施对鸡血藤黄酮类化合物积累的影响

Effect of Zn, B Fertilization on Flavonoids Accumulation in Spatholobus suberectus Dunn.

摘要:

为探讨Zn、B配施对鸡血藤（Spatholobus suberectus）黄酮类化合物积累的影响，采用营养液叶面喷施，对其总黄酮含量、可溶性蛋白质和PAL活性的变化进行研究。结果表明，Zn、B配施的鸡血藤总黄酮含量、可溶性蛋白质含量、PAL活性增加，其中施用50 mg L^-1 ZnSO₄+10 mg L^-1 Na₂B₄O₇·10H₂O鸡血藤的可溶性蛋白质含量最高，达0.89%；施用25 mg L^-1ZnSO₄+50 mg L^-1 Na₂B₄O₇·10H₂O鸡血藤的总黄酮含量和PAL活性最高，分别为4.65%、29.47 U g^-1 min^-1。因此，合理配施Zn、B能促进鸡血藤黄酮类化合物的积累。

Abstract:

In order to understand the effects of Zn, B fertilization on flavonoids accumulation in Spatholobus suberectus Dunn., the changes in contents of flavonoid and soluble protein, PAL activity were studied by using foliage spray. The results showed that the soluble protein content, PAL activity and total flavones content of S. suberectus increased spraying Zn, B fertilization. The soluble protein content was the highest for 0.89% treated with 50 mg L^-1 ZnSO₄ +10 mg L^-1 Na₂B₄O₇·10H₂O. The total flavones content and PAL activity were the highest, for 4.65% and 29.47 U g^-1 min^-1, respectively, when S. suberectus were treated with 25 mg L^-1 ZnSO₄+50 mg L^-1 Na₂B₄O₇·10H₂O. Therefore, reasonabe fertilization of Zn, B could promote the accumulation of flavonoids in S. suberectus.