入侵植物猫爪藤体外细胞毒活性成分的筛选分离

Screening and Isolation of Cytotoxicity in vitro from Invasive Plant Macfadyena unguis-cati L. (Bignoniaceae)

摘要:

为了筛选分离入侵植物猫爪藤的细胞毒活性成分，采用MTT法以75%乙醇提取物的不同组分分别处理人肝癌细胞SMMC7721、Bel7402和正常肝细胞Chang Liver，对他们的体外增殖抑制率进行了研究。结果表明，总醇提物的氯仿组分对肝癌细胞表现出明显的体外增殖抑制作用，其次是石油醚组分。从氯仿萃取组分中分离出具有更强细胞毒活性的成分熊果酸。因此，入侵植物猫爪藤具有体外细胞毒活性，熊果酸是其体外细胞毒活性的主要成分之一。

Abstract:

In order to screen and isolate the active constituents from invasive plant Macfadyena unguis-cati L. (Bignoniaceae), the anti-proliferative effects of SMMC7721, Bel7402 by different solvent extraction fractions of ethanol extracts of M. unguis-cati in vitro were studied by using MTT assay. The results showed that the chloroform fraction of ethanol extracts had strong inhibitory effect on liver cancer cells, and then was petroleum ether fraction. An active constituent isolated from chloroform fraction, ursolic acid, had strong inhibitory effect. Therefore, invasive plant M. unguis-cati L. has cytotoxicity in vitro, and one of the main active components is ursolic acid.