福建省野牡丹属种质资源的ISSR分析

ISSR Analysis of Melastoma Germplasm in Fujian Province

摘要:

为探讨福建省野牡丹属(Melastoma L.)植物的亲缘关系,运用ISSR 分子标记技术对源自福建省的野牡丹属野生种质及部分实生后代共34 份材料进行分析。结果表明,11 条多态性引物对34 份种质DNA 进行扩增,共获得112 条完整、清晰的谱带,其中多态性条带104 条,多态性比率为92.9%,表明野牡丹属种质资源具有较高的遗传多样性。34 份种质材料的相似系数为0.55~0.93,平均相似系数为0.71,表明这些材料间的亲缘关系较近。聚类分析表明34 份材料可划分为3 个类群5 个亚类,主坐标散点分析可分为4 个类群,这与亲缘关系分析的结果基本一致。这从分子水平上揭示了福建省野牡丹属野生种质及部分具备优良园艺性状实生后代的亲缘关系,为该属植物的良种选育工作提供了理论依据。

Abstract:

In order to understand the relationship of Melastoma, the DNA fingerprints of 34 Melastoma germplasm collected from different regions of Fujian Province were studied by using ISSR markers. Eleven primers selected from 116 ISSR primers could obtain high polymorphism and reproducibility bands. The results showed that total of 112 DNA bands were amplified, and 104 polymorphic bands, counting for 92.9%. Genetic similarity analysis showed that for 34 Melastoma germplasm the Nei's coefficient of 34 Melastoma germplasm ranged from 0.55 to 0.93, with the average of 0.71. It was suggested that there were close genetic relationships and high genetic diversity among Melastoma germplasm resources. These 34 germplasm resources could divided into 3 groups and 5 subgroups by UPGMA analysis, and 4 groups by principal coordinate scatter analysis. These results had high consistency with that of genetic relationship analysis. Therefore, it was suggested that ISSR markers could be used to reveal genetic diversity of Melastoma at molecular level, and provided a theoretical basis for breeding of Melastoma in Fujian.