小桐子果壳化感物质的初步分离和鉴定

Preliminary Isolation and Identification of Allelopathic Compounds from Japropha curcas L. Fruit Shells

摘要:

为明确小桐子(Jatropha curcas L.)果壳的化感物质,对小桐子果壳水提物的水萃取物进行了分离和初步鉴定。结果表明,AO活性部位为10 g L^-1时,对萝卜(Raphanus sativus Linn.)、苏丹草[Sorghum sudanense (Piper) Stapf]幼苗生长的抑制率均高于80%。经对AO部位进行HPLC-MS分析,选择鉴定了含量较高的6个化合物,其含量为总量的60.13%。其中,化合物6初步鉴定为楤木皂苷Ⅴ (Araliasaponin Ⅴ),化合物1、2、3、4、5相似,初步鉴定为肽和蛋白,其相对含量之和为56.13%。利用小桐子果壳可开发植物源除草剂。

Abstract:

In order to understand allelopathic compounds from fruit shells of Jatropha curcas L., the aqueous extract were isolated and identified. The results showed that Raphanus sativus Linn. and Sorghum sudanense (Piper) Stapf seedlings were treated with 10 g L^-1 AO active part, inhibition rates of growth were higher than 80%. Six compounds in AO were identified by HPLC-MS, accounting for 60.13% of the total content. The compound 6 was identified primarily to be Araliasaponin V, the other five compounds were similar and identified primarily to be peptide or protein compounds, which the sum of relative content was 56.13%. So, it was suggested that the fruit shells of J. curcas could be used to develop botanical herbicide.