巴西橡胶树HbMyb1基因的原位PCR物理定位

Chromosome Location of HbMyb1 Gene from Hevea brasiliensis by in situ PCR

摘要:

为探讨巴西橡胶树(Hevea brasiliensis)死皮病抗性相关HbMyb1 基因的定位,采用所建立的橡胶树染色体原位PCR 技术体系进行研究。结果表明, HbMyb1 基因初步定位于巴西橡胶树‘热研7-33-97’的第5 号染色体长臂上,信号位点到着丝粒的百分距离为15.21,并观察到在巴西橡胶树‘热研7-33-97’叶片细胞核的不同分裂时期均扩增到1~2 个信号。同时对染色体标本的制备、保存、预处理等方面进行了探讨。

Abstract:

The in situ PCR technical system on chromosomes of Hevea brasiliensis was developed successfully. The HbMyb1 gene related with Tapping Panel Dryness (TPD) resistance was physically located in the long arm of chromosome No. 5 of ‘Reyan 7-33-97’ by in situ PCR and the fraction-length, the percentage of the distance from detection site to the centromere, was 15.21. One or two signals were detected during cell division at different phases. The chromosome preparations, preservation, and pretreatment were also discussed.