转Bt 基因甘蔗抗虫性及重要经济、农艺性状的评价

Insect Resistance and Evaluation on Important Agronomic and Economic Characters of Bt-transgenic Sugarcane Lines

摘要:

为评价转Bt 基因甘蔗的抗虫性,对6 份转Bt 甘蔗品系(Y2、Y3、Y4、T1、T2 和T3)进行室内抗虫性鉴定实验和田间自然感虫的抗虫性评价。结果表明,室内人工抗虫性鉴定结果与田间自然感染结果大体吻合,各参试材料在两个试验点的抗虫性表现基本一致,Bt 基因表达稳定,所有参试品系抗虫性均明显比相应的供体品种强,其中Y4 和T2 的抗性最强,T3 相对较弱;在经济性状方面,Y3、Y4 和T3 的蔗茎产量、含糖量与其供体品种‘新台糖16 号’相当,但甘蔗蔗糖含量较低;T2 的甘蔗蔗糖含量则超过其供体品种‘新台糖16 号’,但农艺性状较差,茎产量和含糖量低。

Abstract:

The insect resistances and evaluations on important agronomic and economic characters of 6 Bt-transgenic sugarcane lines, such as Y2, Y3, Y4, T1, T2 and T3, were studied both in the field and room. The results showed that the insect resistances of these Bt-transgenic sugarcane lines in the field were similar to those in the room, and the Bt gene expression was stable. All of these tested lines had stronger insect-resistance than their wild types, in which Y4 and T2 lines were the strongest and T3 line was the weakest. The cane yield and sugar content in Y3, Y4 and T3 lines were similar to those in their relative original lines, but the sucrose content of these transgenic lines was lower than that in original lines. In addition, although T2 line had higher sucrose content than original line ‘ROC16’, its main agronomic traits were worse with low cane yield and sugar content.