蓝花楹组织培养与快速繁殖研究

Tissue Culture and Rapid Propagation of Jacaranda mimosifolia

摘要:

以蓝花楹(Jacaranda mimosifolia Humb. et Bonpl.)胚轴为外植体进行组织培养和快繁体系建立的研究。结果表明,蓝花楹种子经40℃~45℃温水浸泡后发芽率较高,达到55.7%。蓝花楹不定芽和愈伤组织诱导的最适培养基分别为MS+6-BA 2.0 mg L^-1+NAA 0.1 mg L^-1+2,4-D 0.1 mg L^-1和MS+6-BA 0.5 mg L^-1+NAA 1.0 mg L^-1+2,4-D 1.0 mg L^-1。 不定芽和愈伤组织增殖的最适培养基分别为改良MS培养基+ 6-BA 0.5 mg L^-1 +NAA 0.5 mg L^-1+ IBA 0.5 mg L^-1和MS+6-BA 1.0 mg L^-1+NAA 0.5 mg L^-1+ZT 3.0 mg L^-1。愈伤组织分化最适培养基为MS+BA 1.0 mg L^-1+NAA 0.5 mg L^-1+2,4-D 0.5 mg L^-1。最适生根培养基为1/2MS+蔗糖20 g L^-1+NAA 0.1 mg L^-1+活性炭2.0 g L^-1,生根率达78.3%。

Abstract:

The hypocotyls of Jacaranda mimosaefolia used as explants, the tissue culture and rapid propagation system were studied. The results showed that the seed germination rate of J. mimosaefolia reached up to 55.6% after warm water immersion for 60 minutes at 40℃-45℃. The optimum mediums for induction of adventitious buds and callus were MS+6-BA 2.0 mg L^-1+NAA 0.1 mg L^-1+2,4-D 0.1 mg L^-1, and MS+6-BA 0.5 mg L^-1+NAA 1.0 mg L^-1+2,4-D 1.0 mg L^-1, respectively. The optimum mediums for proliferation of adventitious buds and callus were modified MS+6-BA 0.5 mg L^-1+NAA 0.5 mg L^-1+IBA 0.5 mg L^-1, and MS+6-BA 1.0 mg L^-1+ NAA 0.5 mg L^-1+ZT 3.0 mg L^-1, respectively. The optimum medium for callus differentiation was MS+6-BA 1.0 mg L^-1+NAA 0.5 mg L^-1+2,4-D 0.5 mg L^-1. The optimum rooting medium was 1/2MS supplemented with 20 g L^-1 sugar and NAA 0.5 mg L^-1 and 2.0 g L^-1 active carbon (AC) with rooting up to 78.3%.